Salmonella tests have similar efficacy
A recent study of methods for detecting Salmonella
indicated that the Reveal test and the US Food and Drug Administration (FDA)
culture method are of similar efficacy.
is a leading cause of foodborne illnesses, and
(SE) is a frequently isolated Salmonella
serovar, with egg products often associated with outbreaks.
To prevent SE contamination of eggs, many producers are implementing flock
inspections for SE at their facilities. A rapid and simple method for detecting
SE in poultry environmental samples is critical for effective control of SE.
In this study, the Reveal test for SE was compared with the conventional FDA
culture method for detecting
SE in naturally contaminated environmental samples.
One hundred twenty-eight environmental drag swab samples were collected
from two previously identified SE-positive chicken flocks in two US states and
analysed in parallel using the Reveal test and the FDA culture method.
Twenty-five samples (19.5%) yielded SE when the Reveal test
and 23 samples (18.0%) were positive for SE by the FDA culture method. No
significant difference in efficacy (P = 0.527) was found between the two
The efficacy of two enrichment media, tetrathionate broth (TT) and
Rappaport-Vassiliadis medium (RV), and three selective plating media, brilliant
green agar with novobiocin (BGN), xylose lysine tergitol 4 agar (XLT4), and
bismuth sulfite agar (BS), also were compared for SE isolation.
Overall, a significantly greater number of positive samples was obtained
after enrichment in RV compared with TT. XLT4 and BGN were more efficient than
BS for isolating SE.
However, no single method or medium successfully recovered SE from all
SE-positive environmental samples.
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